Brits are still allowed to travel to 19 European countries without the need to quarantine on their return to the UK.
A surge in coronavirus cases in the continent has led to the Foreign Office now removing France and the Netherlands off its safe list.
However, the likes to Greece and Germany remain, despite major spikes.
On Wednesday Greece reported 262 new cases, its highest daily tally since the start of the pandemic, reports The Mirror.
Authorities in the Mediterranean country have introduced new measures as a result of the increase in recent weeks, including a curfew for restaurants and bars.
The full list of 19 countries is:
Arrivals from multiple EU and Balkan nations also have restrictions.
Germany, meanwhile, recorded its biggest daily increase in cases in more than three months on Wednesday with 1,200.
On the same day, France had 2,524 new cases in 24 hours, the highest increase since May when lockdown was lifted.
The removal of France came after Boris Johnson said ministers would be "absolutely ruthless" in deciding on whether to impose the self-isolation requirement.
The Netherlands, Monaco, Malta, Turks & Caicos and Aruba have also been added to the quarantine list from 4am on Saturday.
The decision to add France will cause dismay for thousands of British holidaymakers currently in the country.
It was made in response to the spread of the virus, with the latest 14-day cumulative figures showing 32.1 Covid-19 cases per 100,000 people in France, compared with 18.5 in the UK.
The move will also come as a bitter blow to the hard-pressed French tourism industry which relies heavily on visitors from the UK.
Meanwhile France's secretary of state for European affairs Clement Beaune said the UK decision would lead to "reciprocal measures" across the Channel.
With Spain already on the quarantine list, it now means that holidaymakers returning to the UK from the two most popular overseas destinations must now isolate for 14 days.
The timing is set to trigger a race for Channel ferry ports, airports and the Eurotunnel as thousands of desperate tourists try to avoid self-isolation.
Meanwhile, lockdown measures in England will be further eased this weekend.
Facial beauty services, tattoos and spa treatments can resume, Johnson has announced.
Wedding receptions with up to 30 guests can take place, trials of sporting events with a limited number of spectators can restart and indoor performances at theatres, comedy clubs and concert venues with socially distanced audiences can begin again.