Two MPs from Greater Manchester were among just 23 who voted against the new requirement for face coverings to be worn in shops and on public transport.
Sir Graham Brady, the Conservative member of parliament for Altrincham and Sale West, and Chris Green, MP for Bolton West, were the only two from Greater Manchester who were not in favour of the face coverings.
The pair also voted against the new self-isolation regulations introduced, forcing all travellers returning to the UK to take a PCR test and self-isolate until they receive a negative result.
Sir Brady said there were "serious concerns" about the "efficiency of what is being proposed."
READ MORE:'This is our life now, we just have to get on with it': How do passengers at Piccadilly station feel about wearing masks again?
Those who voted against masks were hugely outweighed by the 434 MPs who voted in favour of the new guidance.
And just 36 voted against the new self-isolation rules, compared with 431 who were in favour of the restrictions.
The vote comes as people were ordered to wear masks again in shops and on public transport from Tuesday, November 30.
The Prime Minister faced a backbench backlash as the House of Commons considered the requirement for face coverings to be worn in shops and public transport in England.
Conservative MPs Philip Hollobone (Kettering) and Craig Mackinlay (South Thanet) acted as the tellers for the noes on both votes.
According to PA, Sir Graham Brady, who also chairs the Conservative backbench 1922 Committee, said there were “serious concerns” about the “efficacy of what is being proposed”, and warned against “mission creep”.
He added: “I think we should all be afraid of the madness of that kind of policy.
“And the difficulty is that, maybe 18 months ago, when some of us started raising these concerns, it was possible for some people to suggest that we were being fanciful.
"We’ve now lived it for 18 months, and we can see this reaching ahead.
“Now again we see the Government’s immediate assumption that what it should reach for is new controls, new compulsion.
"New rules that will be inflicted on the British people.
“And I think we need to move away from that, move back to a world where we trust people.”
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Following the decision, Boris Johnson was accused of introducing a “scaremongering propaganda campaign” to restrict social interaction, as Conservative MPs criticised new measures to combat the Omicron variant of coronavirus.
Former minister Sir Christopher Chope labelled the regulations “oppressive, authoritarian and dictatorial” as he warned it would have an “adverse effect” on lives and livelihoods.
He argued ministers had produced “no evidence whatsoever” to show the impact of the regulations on protecting public health.
Opening the debate on Tuesday afternoon, MP Ms Maggie Throup said the new measures were “proportionate, precautionary and balanced”.
She said: “While we are not implementing the entirety of the (autumn and winter) plan now, we are taking steps to respond to a potentially potent mutation of the virus.”
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