Three quarters of nightlife and hospitality businesses say they'll be out of business by Christmas under the tougher tier system.
The majority of the country will be placed into tightened Tier 2 or 3 measures when the national lockdown is lifted on Wednesday.
Restaurants and pubs in Tier 2 areas will only be able to serve alcohol alongside substantial meals, while in Tier 3 regions including Greater Manchester, they must close altogether and may only operate as takeaways.
Meanwhile nightclubs have been unable to open since March.
Wet-led pubs have been promised an extra £1,000 each to help them through December, Boris Johnson announced today - but the cash has been dismissed as 'an insulting token gesture'.
The Night Time Industries Association has accused the Government of 'intentionally aiming to collapse our sector' with the new tier system, which MPs are voting on on Tuesday evening.
In a survey of 400 of its members, 75.6 per cent said they will be closed for good by Christmas.
Nearly two thirds (65 per cent) have already made over 40 per cent of their workforce redundant, the findings also revealed.
The NTIA is warning hundreds of thousands more jobs will be lost over the Christmas period and into January as restrictions over the festive period 'break the back of thousands of businesses'.
Michael Kill, CEO, said: "This announcement by the Government has led us to believe that they are intentionally aiming to collapse our sector.
"Every town and city across the UK stands to lose valued and much loved venues. This will be another stab in the heart of our town and city centres.
“We stand to lose the cultural institutions and amazing workforce of professionals that the UK are renowned for globally.
"Our clubs, bars, venues, security, freelancers, staff, managers, DJs and many more will lose their livelihoods and continue to suffer financial hardship without government intervention.
“I make a direct appeal to the Prime Minister - ‘Mr Johnson, what are you doing to save the lives and livelihoods of the many businesses and workers within the night time economy, businesses that have been closed since March and are continuing to suffer?
"They have staff and freelancers that will lose their jobs irrespective of furlough because the businesses won’t survive’.
“What do you say to that Prime Minister? I hope you are sleeping well at night because thousands within our sector are struggling to sleep, in fear of their future.”