Jet2 is advising passengers against wearing face masks when flights resume in July.
This is in contrast to Ryanair, which has made face coverings mandatory, and to Easyjet, which is recommending them.
A statement on the Jet2 website states: "Public Health England (PHE) advises against using masks outside clinical settings.
"PHE state that if you’re fit and well, with no symptoms, there’s no evidence that wearing face masks as a general prevention measure is helpful."
The budget airline is deep cleaning aircraft and transfer coaches on a daily basis, and says: "The health and safety of our customers is our number one priority and we’re taking all appropriate measures."
Many other European airlines, including Ryanair, KLM and Iberia, have made face masks mandatory both in airports and on board planes.
Passengers without a mask or suitable face covering will not be allowed to board the aircraft, and they must be worn for the duration of the flight.
Other airlines, including Easyjet and TAP Portugal, have not made face masks mandatory - but are recommending them to passengers and staff.
Jet2 will resume its flight programme on 1 July 2020 after grounding flights as a result of the coronavirus pandemic.
While the FCO still advises against all but essential foreign travel, UK airlines are preparing for a time when they get the green light.
From 8 June anyone arriving in the UK will have to quarantine for 14 days, or risk paying a hefty fine.