Brits returning from Belgium, the Bahamas and Andorra will have to self isolate for 14 days under new quarantine rules.
The Department for Transport confirmed it was axing quarantine-free travel with the three popular holiday destinations from 4am on Saturday following spikes in coronavirus cases.
Data from the Joint Biosecurity Centre and Public Health England found a "significant change in both the level and pace of confirmed cases of coronavirus in all three destinations" - leading to their removal from the Government's list of travel corridors.
The guidance applies across the whole of the UK, with Wales announcing the news first on Thursday evening, followed by Scotland and England.
However quarantine comes in from midnight on Friday for Welsh tourists - instead of Saturday as for England and Scotland.
The Foreign Office is now advising against all but essential travel to the three countries.
In a bit of good news for travellers, Malaysia and Brunei have been added to the travel corridors list for England.
From 4am on Tuesday, holidaymakers from both countries will be able to return to England without needing to quarantine.
However Portugal remains on the "red list" - despite speculation that the Government could be preparing to ease the quarantine restrictions.
Grant Shapps, the Transport Secretary, tweeted: "Data shows we need to remove Andorra, Belgium and The Bahamas from our list of #coronavirus Travel Corridors in order to keep infection rates DOWN.
"If you arrive in the UK after 0400 Saturday from these destinations, you will need to self-isolate for 14 days."
In Andorra, new cases per week have increased five-fold since mid-July, while in The Bahamas the weekly case rate peaked at 78.6 last week, up from 3.1 in mid-July.
Belgium has seen a surge in coronavirus cases in recent weeks, rising to 27.8 new cases per 100,000 people on Thursday,
It now has one of the highest coronavirus mortality rates in the world, with 84 deaths per 100,000 people.
The Belgian Government has imposed new measures to curb the spread of the virus, including a ban on people meeting more than five others outside of their household.
As many as half of the cases have been in the city of Antwerp, where a night-time curfew has been imposed.
Infection rates in Belgium are now higher than they were in Spain when the UK reimposed quarantine measures - when the number of cases was 27.4 per 100,000.
The Government sparked a diplomatic row with Madrid when they axed the so-called travel corridor on July 25 with only a few hours notice, causing chaos for holidaymakers and the travel industry.
Mr Shapps was caught out by his own department’s quarantine rules after flying to Spain for a family holiday only hours before the change was announced.
He was forced to return to the UK to deal with the chaos - and to self isolate at home for two weeks, in line with the new Government guidance.
Luxembourg was also added to the list last week after a spike in case.
Mr Shapps previously warned holidaymakers that he “cannot rule out” adding other destinations to the quarantine list as the Government eyes the infection rates in Europe with growing concern.