Arrivals into the country from Belgium, Andorra and the Bahamas will have to quarantine from 4am on Saturday.
Transport Secretary Grant Shapps confirmed restrictions would apply to travellers from the three countries on Twitter.
Since July 10, UK residents have been able to travel to more than 70 countries without needing to self-isolate when returning.
But since then, the list has been getting gradually smaller. Spain was dramatically removed and Luxembourg has also been struck off.
Now three more countries are being taken off, meaning anyone returning from those nations after Friday will need to stay at home for a fortnight.
In a tweet he said: “Data shows we need to remove Andorra, Belgium and The Bahamas from our list of #coronavirus Travel Corridors in order to keep infection rates DOWN.
“If you arrive in the UK after 0400 Saturday from these destinations, you will need to self-isolate for 14 days.”
The move is in response to rising infection figures in each of the three countries.
Returning passengers will be asked to provide contact details and random spot checks can be carried out to ensure compliance.
Fines of up to £1,000 can be dished out for those breaking the rules.
The change is being made UK wide. Scottish Justice Secretary Humza Yousaf said: “The governments of all for nations have agreed to these changes based on a shared understanding of the data.
“This is another important step in our efforts to prevent the spread of the virus, and adhering to the quarantine is a vital aspect of this.
“Imposing quarantine requirements on those arriving from another country is not a decision made lightly – but suppressing the virus and protecting public health remains our priority.”