Paris Saint Germain superstar Neymar lasted just 12 minutes in the friendly in Singapore, before being substituted.
The 27 year-old, who has endured an injury hit 18 months, appeared to suffer a hamstring injury in the early throes of the 1-1 draw.
He was subsequently replaced by Philippe Coutinho.
The blow is the latest in a series of issues for Neymar, who has only featured for PSG five times this season.
He also missed Brazil's Copa America victory during the summer.
After the game, Tite was quizzed over whether he had taken an unnecessary risk playing the forward, who was winning his 101st international cap.
But Tite insisted he would not have played his star man if there had been any pre-game doubts over his fitness.
"No, not at all," Tite replied when asked if Neymar was a risk before the game.
"It would be irresponsible and incompetent of me. I would have to receive the complaints of the coach and the president of PSG if that happened.”
PSG are back in action on Friday night, when they face Nice in Ligue 1.
They will be back in Champions League action the following week, although Neymar still has one game of a three-match European ban remaining.
Liverpool goalkeeper Alisson isn't with the Brazil squad having only just returned from injury.
But he believes that Neymar could still land the Ballon d'Or, the individual award he continues to long for.
“Neymar is a high-level player and would be fundamental in any team in the world," he told Spanish media.