Gutted Borna Barisic suffered a blow to his Euro 2020 hopes after he missed Croatia's opener against England.
The Rangers star was expected to start for his country at Wembley having started the last Euro 2020 warm-up match against Belgium.
He was replaced at half-time by Josko Gvadiol and the Dinamo Zagreb kid impressed following the break.
Question marks had the surround Zlatko Dalić's decision with the first-choice left-back missing out on the 23-man squad completely.
However, it now appears that niggling injuries are keeping the Ibrox favourite out of the squad
Dalic told HRT : "Barisic has had problems with injuries for the last ten days, Guardiol is now the most prepared."
Barisic had missed the final two league games of the campaign having picked up a knock in the final clash of the season against Celtic.
He was forced off in the closing stages of Rangers ’ 4-1 derby day win with the countdown but has played two warm-up friendlies for his national side.
It is now a waiting game to see if he will be fit to face the Czech Republic and Scotland in the final Group D game at Hampden.